Abstract
Novel properties of a previously obtained Bacillus subtilis 26DCryChS strain are described. The B. subtilis 26DCryChS strain is able to produce Cry1Ia δ-endotoxin from B. thuringiensis B-5351 and to exist in internal plant tissues of potato plants in the same manner as the endophytic B. subtilis 26D source strain (487 ± 53 and 420 ± 63 CFU*103/g, respectively). B. subtilis 26DCryChS, as much as the original B. subtilis 26D strain, inhibited mycelium growth of oomycete Phytophthora infestans (Mont.) de Bary and reduced late blight symptoms development on plants by 35% compared with non-treated ones, as well as showed insecticidal activity against Leptinotarsa decemlineata. Production of the fluorescent GFP protein in the B. subtilis 26D genome allowed visualizing the endophytes around damaged sites on beetle intestines. Bacillus strains under investigation induced systemic resistance to P. infestans and L. decemlineata through the activation of the transcription of PR genes in potato plants. Thus, the B. subtilis 26DCryChS strain was able to induce transcription of jasmonate-dependent genes and acquired the ability to promote transcription of a salicylate-dependent gene (PR1) in plants infected with the late blight agent and damaged by Colorado potato beetle larvae. The B. subtilis 26DCryChS strain could be put forward as a modern approach for biocontrol agents design.
Highlights
IntroductionIt is estimated that 35% of agricultural plants’ yield is lost due to biotic damage (insects, weeds, and pathogens) in the field around the world
It is estimated that 35% of agricultural plants’ yield is lost due to biotic damage in the field around the world
The content of B. thuringiensis B-5351 cells in potato plants was substantially lower than the B. subtilis strains under investigation
Summary
It is estimated that 35% of agricultural plants’ yield is lost due to biotic damage (insects, weeds, and pathogens) in the field around the world. Postharvest losses may constitute up to 15%. Of the total yields [1]. This has resulted in the complete dependence of crops on the use of chemical pesticides to reduce losses. Target organisms often develop resistance to globally used chemicals. The most limiting productive capacity factor for potato is late blight caused by Phytophthora infestans (Mont.) de Bary infection. Late blight causes massive yield losses annually, especially under favorable conditions for P. infestans dispersion [2]. Since the last quarter of the century, different fungicides have been developed for the management of potato late blight disease, but the number of P. infestans isolates resistant to fungicides continuously increases
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