Abstract
The endogenous production of pregnenolone and testosterone in subcellular fractions of rat testis tissue was estimated. A medium containing cofactors was used and the incubations were carried out for 120 min at 33 °C. Only mitochondrial fractions produced the estimated steroids. Seminiferous tubules and interstitial tissue were isolated from rat testis tissue using a wet dissection technique. Mitochondrial fractions were isolated from the homogenized isolated tissue compartments and production of pregnenolone and testosterone after incubation of these fractions was estimated. The interstitial mitochondrial fraction produced 1200–2600 pmoles per mg mitochondrial protein per 2 h which was at least 60 times higher than the production in similar fractions obtained from seminiferous tubules. Production in the mitochondrial fraction of whole testis tissue was 300–600 pmoles per mg protein per 2 h.
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