Abstract
Abstract Ethanolic extracts of sour cherry (Prunus cerasus L. cv. Montmorency) fruit were partitioned into butanol and water-soluble fractions. Following paper chromatography, each fraction was tested for cytokinin activity by the radish cotyledon and soybean callus bioassays. Activity in both fractions increased between 14 and 21 days after anthesis, and remained high at 28 days, although cell division in the pericarp had ceased. One growth promotor in the butanol fraction resembled zeatin in its chromatographic mobility on both paper and Sephadex LH-20, and was identified as zeatin by gas chromatography-mass spectrometry of its trimethylated derivative. A water-soluble growth promoter, which exhibited activity in only the radish cotyledon bioassay, was unaffected either by acid or by alkaline phosphatase hydrolysis.
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