Abstract
Endogenous sinapic acid (SA), sinapine (SP), sinapoyl glucose (SG) and canolol (CAN) of canola and mustard seeds are the potent antioxidants in various lipid-containing systems. The study investigated these phenolic antioxidants using different fractions of canola and mustard seeds. Phenolic compounds were extracted from whole seeds and their fractions: hulls and cotyledons, using 70% methanol by the ultrasonication method and quantified using HPLC-DAD. The major phenolics from both hulls and cotyledons extracts were SP, with small amounts of SG, and SA with a significant difference of phenolic contents between the two seed fractions. Cotyledons showed relatively high content of SP, SA, SG and total phenolics in comparison to hulls (p < 0.001). The concentration of SP in different fractions ranged from 1.15 ± 0.07 to 12.20 ± 1.16 mg/g and followed a decreasing trend- canola cotyledons > mustard cotyledons > mustard seeds > canola seeds > mustard hulls > canola hulls. UPLC-tandem Mass Spectrometry confirmed the presence of sinapates and its fragmentation in these extracts. Further, a high degree of correlation (r = 0.93) was noted between DPPH scavenging activity and total phenolic content.
Highlights
Over the last two decades, the positive effects of endogenous bioactive phenolic compounds from plants and oilseeds have received considerable attention due to the role of phenolic antioxidants in human nutrition and health [1,2]
The results might not be very suitable for comparisons as the authors used an alkaline treatment method which might have affected the structural attribute of the original phenolics and thereby, its quantification
With respect to the ionization of phenolic compounds from mustard and canola fractions, our study indicated that a positive mode is best suited for detecting
Summary
Over the last two decades, the positive effects of endogenous bioactive phenolic compounds from plants and oilseeds have received considerable attention due to the role of phenolic antioxidants in human nutrition and health [1,2]. There are no data on the in-situ distribution of phenolics in different sections of an oilseed, canola or mustard This information will further strengthen the rationale of value addition of oilseed by-products such as hulls, meals, and press cakes for recovering phenolics. In this scenario, the purposes of our study were to identify, quantify, and characterize the antioxidant phenolics of hulls and cotyledons of canola and mustard seeds. The major phenolics were characterized using ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS), and the potential DPPH scavenging of these various residues of canola and mustard seeds was investigated This knowledge is essential to contribute to the optimized extraction of canolol from sinapic acid and other precursors
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
