Abstract
A capillary electrophoretic method for enantiomeric separation of five β-agonists was established. The effects of the chiral selector type and concentration, pH value, composition of the background electrolyte (BGE), the capillary temperature, applied voltage, and capillary length on the enantiomeric separation of the drugs selected have been investigated. The results showed that the chiral selector type and concentration and pH value have strong influence on the efficiency of the chiral separation. The enantiomers of all drugs examined were completely resolved with carboxymethyl-β-cyclodextrin (CM-β-CD). Optimal separation was obtained using a running buffer of 50 mM phosphoric acid containing 10 mM CM-β-CD at pH 3.5 in 48.5 cm × 75 µm (40 cm effective length) uncoated capillary. The capillary temperature was at 15°C and applied voltage was at 20 kV.
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