Enantioselective intramolecular C-H amination catalyzed by engineered cytochrome P450 enzymes in vitro and in vivo.

Abstract

Nitrogen activation: Though P450 enzymes are masters of oxygen activation and insertion into CH bonds, their ability to use nitrogen for the same purpose has so far not been explored. Engineered variants of cytochrome P450BM3 have now been found to catalyze intramolecular CH aminations in azide substrates. Mutations to two highly conserved residues significantly increased this activity.