Emergence of Axonal Tracts in the Developing Brain of the Turbot (Psetta maxima)

Abstract

In this study we have investigated the pattern of morphogenesis and axogenesis in the turbot brain during embryonic and early larval stages with immunohistochemistry using an antibody against acetylated tubulin. The first immunoreactive elements were detected at 74 h post-fertilization in fibers running in the medial and lateral longitudinal fascicles. Newly positive axonal bundles are progressively added during development forming rostrocaudally directed tracts. The tract of the postoptic commissure appears at 86 h post-fertilization located rostrally to the medial longitudinal fascicle. Together, the medial longitudinal fascicle and the tract of the postoptic commissure constitute a major longitudinal axonal pathway, which is extended rostrally in embryos of 98 h post-fertilization by the supraoptic tract. In the forebrain, two vertical tracts, the tract of the posterior commissure (appearing around 98 h post-fertilization) and the tract of the anterior commissure (detected at 110 h post-fertilization) project descending axons to the pre-existing axonal longitudinal pathway. These early tracts are connected by four associated commissures (ventral tegmental, postoptic, posterior and anterior commissure). Some groups of labeled cell bodies are identified either as the origin of the embryonic tracts or contributing axons to the axonal pathways. Additionally, a conspicuous cluster of large cells, not clearly associated with any axonal bundle, was observed from 98 h post-fertilization lining the caudal floor of the presumptive hypothalamus. Several hypotheses are proposed to determine the nature of these cells. A comparison of the emergence of the axonal circuitry in turbot and that of other teleosts reveals significant analogies, suggesting that a common pattern underlies the establishment of the embryonic tracts in this vertebrate group. The minor differences observed between different teleost species, associated with the absence of some axonal fascicles, is also considered.