Embryo production and transplantation in non-breeding season of meat sheep breeds by stimulating superovulation with different follicle-stimulating hormone preparations.

Abstract

The objective of this study was to investigate the response of Charolais and Ile-de-France meat sheep breeds to stimulate superovulation with various follicle-stimulating hormone (FSH) preparations. A total of 14 adult ewes of meat sheep breeds were used in our study as donors, including Charolais breed (n=8) and Ile-de-France breed (n=6). Donors ewes were randomly divided into two groups in equal numbers (first group, n=7; second group, n=7), every group included Charolais breed (n=4) and Ile-de-France breed (n=3). Ewes in the first group were treated with Folltropin-V (total dose of 200 mg per ewe, seven injections), and ewes in the second group were treated with FSH-P (total of 280 IU per ewe, six injections). Thirty-seven ewes of Edilbay breed used as recipients were divided into two groups (first group, n=20; second group, n=17). Our results showed that the number of corpora lutea in donor groups treated with Folltropin-V was significantly higher than in donor groups treated with FSH-P (p < .01). A greater number of embryos recovery and embryos suitable for transplantation were found in the first group compared with the second group of donors. After 30 days from transplantation, transabdominal ultrasonography showed that the presence of pregnancy in recipients groups was found in 16 recipient ewes (43.2%), in the first group of recipients were registered nine pregnant ewes of 20 recipient ewes (45.0%), and in the second group of recipients were registered seven pregnant ewes of 17 recipient ewes (41.2%). In conclusion, using Folltropin-V in Charolais and Ile-de-France meat sheep breeds is a more effective scheme for stimulating superovulation than using FSH-P.