Abstract
The dehydrogenase system of Saccharomyces cerevisiae was electrostimulated utilizing Helmholtz coils (average 4 mT, 60 Hz) to induce a current density of microamps per square centimetre or a field strength of the order of millivolts per centimetre. As an indicator, the reduction rate of methylene blue (MB) was used, measured by its polarographic signal instantaneously in the range of minutes. Up to now the effect of field on the reduction yield of MB has been determined depending on the following parameters: the concentration of cells in suspension, the pre-exposure time before adding MB to the cells, the temperature of the solution, the influence of the physiological state of growing cells and the concentration of phosphate buffer. Non-linear responses were detected in some of these dependences. As a general rule one can conclude that the slower the reduction rate, the higher the stimulation effect. The combination of MB with the dehydrogenase system turns out to be a method for determination of the electromagnetic field effect. This technique may also be useful for other microorganisms.
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