Abstract
In this study we have reported the design and development of a facile, sensitive, selective, and label-free electrochemical sensing platform for the detection of atrazine based on MWCNT-embedded ZnO nanofibers. Electrospun nanofibers were characterized using scanning electron microscope (SEM), transmission electron microscope (TEM), X-ray diffraction (XRD), X-ray photoelectron spectroscope (XPS), UV-Visible spectroscope (UV-VIS), and Fourier-transform infrared spectroscope (FTIR). Electrochemical properties of MWCNT-ZnO nanofiber-modified electrodes were assessed using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). Binding event of atrazine to anti-atrazine antibody, which immobilized on nanofiber-modified electrode via EDC and NHS chemistry, was transduced with EIS. Due to high conductivity, surface area, and low bandgap of MWCNT-ZnO nanofibers, we have achieved the sensitivity and limit of detection (LoD) of sensor as 21.61 (KΩ μg−1 mL−1) cm−2 and 5.368 zM for a wide detection range of 10 zM–1 µM. The proposed immunosensing platform has good stability, selectivity, repeatability, and reproducibility, and are less prone to interference.
Highlights
Pesticide is the mixture of organic substance used to prevent, destroy, or migrate any pest
In case of electrochemical immunosensors, low ranges of detection are often explained in terms of heterogeneity of the bioelectrode
On the other hand, electrode current density exponentially depends on charge transfer coefficient, which inherently depends on activation barriers
Summary
Pesticide is the mixture of organic substance used to prevent, destroy, or migrate any pest. Atrazine (1-chloro-3ethylamino-5-isopropylamino-s-triazine; ATZ) is the most widely used pesticide of the triazine family, in crops due to its high efficiency[1,2]. Exposure to ATZ during maternity period results in low fetal weight and limb/urinary/heart defects, with the risk of reduced survival on prolong exposure to high-level concentrations. According to the US environmental protection agency, the maximum acceptable level of ATZ concentration in drinking water is 3 parts per billion[9], long-term exposure to such low concentrations effects the human endocrine system severely. On account of the adverse effects of ATZ on environment, it is desirable to develop biosensor platforms that can detect the same in water, both qualitatively and quantitatively
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
