Abstract
Platelet-rich fibrin (PRF) as a reservoir of various growth factors plays an essential role in wound healing and tissue engineering at present. Electrospinning technology is an efficient approach to acquire artificial scaffold which has large specific surface area and high porosity. The goal of this study was to investigate the potential of electrospinning on the proliferation and osteogenesis of osteogenic precursor cells in vitro, with lyophilized PRF added as a component for electrospinning preparation. The surface structure of lyophilized PRF and nanofibers were investigated, and the proliferation, osteogenesis of MEC3T3-E1 cells with lyophilized PRF or nanofibers extract were studied. The results showed that the diameters of the lyophilized PRF pores were 1.51 ± 0.75 μm, and lyophilized PRF medium promoted the proliferation and osteocalcin (OCN) and osteopontin (OPN) genes expression of MEC3T3-E1 cells. Furthermore, the diameters of the polyvinyl alcohol/sodium alginate/lyophilized PRF (PVA/SA/PRF) fibers were 201.14 ± 40.14 nm. Compared to PVA/SA nanofibers extract and control medium, PVA/SA/PRF nanofibers extract also enhanced the proliferation and mineralization activity of MEC3T3-E1 cells. These results might be instructive to future therapeutics with PVA/SA/PRF electrospinning for bone tissue engineering or other applications.
Highlights
Platelets are rich in various bioactive factors and present excellent potential in tissue regeneration
The fast Fourier transform (FFT) analysis showed that gray value of spectral intensity distribution range went from 55,400∼60,000 to 60,400∼65,700. These results indicated that lyophilized Platelet-rich fibrin (PRF) added as a component of the electrospinning material modified the physical properties of the nanofibers, especially the surface characteristics, which might be important for bone tissue engineering
The results showed that polyvinyl alcohol (PVA)/sodium alginate (SA)/PRF nanofibers extract presented an extraordinary capacity to promote the proliferation and mineralization activity of MEC3T3-E1 cells in contrast to PVA/SA nanofibers extract and control medium, which demonstrated that PVA/SA/PRF nanofibers could maintain the bioactivity of proliferation and osteogenesis-promoting cytokines, and release them to the medium
Summary
Platelets are rich in various bioactive factors and present excellent potential in tissue regeneration. PRF has been demonstrated to facilitate the regeneration of bone, periodontal tissue, and dental pulp (Chang and Zhao, 2011; Ji et al, 2015; You et al, 2019). Either used as a scaffold for tissue engineering or as a medical dressing for clinical application, fresh PRF encountered some limitations for its further application. It is not suitable for storage or transit in liquid nitrogen or on dry ice (Morris, 2005). Previous study demonstrated that, compared to fresh PRF, lyophilized PRF presented better cell proliferation-promoting capacity in vitro, and better histocompatibility and bone regeneration in vivo (Li et al, 2014)
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