Abstract
BACKGROUND:Today’s treatment options for renal diseases fall behind the need, as the number of patients has increased considerably over the last few decades. Tissue engineering (TE) is one avenue which may provide a new approach for renal disease treatment. This involves creating a niche where seeded cells can function in an intended way. One approach to TE is combining natural extracellular matrix proteins with synthetic polymers, which has been shown to have many positives, yet a little is understood in kidney. Herein, we investigate the incorporation of laminin into polycaprolactone electrospun scaffolds.METHOD:The scaffolds were enriched with laminin via either direct blending with polymer solution or in a form of emulsion with a surfactant. Renal epithelial cells (RC-124) were cultured on scaffolds up to 21 days.RESULTS:Mechanical characterization demonstrated that the addition of the protein changed Young’s modulus of polymeric fibres. Cell viability and DNA quantification tests revealed the capability of the scaffolds to maintain cell survival up to 3 weeks in culture. Gene expression analysis indicated healthy cells via three key markers.CONCLUSION:Our results show the importance of hybrid scaffolds for kidney tissue engineering.
Highlights
According to the World Health Organization 10% of the world’s population suffer from acute or chronic kidney diseases [1]
Our results show the importance of hybrid scaffolds for kidney tissue engineering
Average fibre diameter of scaffolds ranges from 1.81 ± 0.24 to 2.83 ± 0.21 lm (Table 1) where emulsion group has the smallest
Summary
According to the World Health Organization 10% of the world’s population suffer from acute or chronic kidney diseases [1]. Drug therapy given to patients has been shown to lead to drug–induced toxicity, and is stated as being responsible for 19–25% of all cases of severe renal failure [3] This paucity of current treatment options for renal failure reveals the need for more reliable and effective methods. One of the endeavours in this field is creating a three-dimensional foundation that can provide a suitable niche for cells to behave as they would in vivo For this purpose, electrospinning of polymers has been widely used to produce fibrous scaffolds as it enables easy manipulation of chemical structure and fibre architecture [6,7,8]. Tissue engineering (TE) is one avenue which may provide a new approach for renal disease treatment This involves creating a niche where seeded cells can function in an intended way. CONCLUSION: Our results show the importance of hybrid scaffolds for kidney tissue engineering
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