Abstract
Since the introduction of electroporation a decade ago, the chick embryo has evolved into a powerful system for studying gene regulation and function during development. Although this is a straightforward method for embryos, skin (especially lamellar tissue) present special challenges. Here we describe a protocol for electroporation of expression vectors into the chick skin explant, with precise spatial and temporal control. We have improved the design of the electroporation chamber, which can achieve precise regulation of the electroporation area by changing the parameters of the laser cutting of the acrylic plate. We give the electroporation parameters of chicken embryo skin, which can effectively improve the electroporation efficiency. This technique can be used for time-lapse imaging and to study gene regulation in chick embryo skin.
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