Abstract

The electrophysiological effects of both trypsin-activated native and 42- and 51-kDa cloned binary toxins ofBacillus sphaericuswere investigated on culturedCulex quinquefasciatuscells using the patch-clamp technique. Rates of reduction in whole-cell membrane resistance were correlated with increasing native toxin concentration. The 42- or 51-kDa cloned toxin alone at 50 μg/ml reduced the resistance. Electrophysiological effects occurred before any changes were visible by phase-contrast microscopy.

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