Abstract
Mechanosensitive (MS) ion channels are membrane proteins that detect and respond to membrane tension in all branches of life. In bacteria, MS channels prevent cells from lysing upon sudden hypoosmotic shock by opening and releasing solutes and water.Specially prepared giant E. coli spheroplasts of bacteria can be used to study the function of bacterial ion channels in patch clamp experiments. Several native mechanosensitive channels can be measured in E. coli. Although this technique has been used since 1987 it still needs a lot of practice to measure native E.coli membranes.View Large Image | View Hi-Res Image | Download PowerPoint SlideThe spheroplasts don't have a Cytoskeleton and are therefore very fragile. using a planar borosilicate substrate to patch these membranes stabilizes these membrane patches.The currents shown above are elicited by increasing pressure steps ranging from 0 to - 120 mbar.A characterization of endogenous mechanosensitive ion channels and overexpressed ones will be shown on the poster.
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