Abstract
An inexpensive Plexiglas apparatus which allows a simple and rapid preparation of horizontal polyacrylamide gels of different dimensions for different purposes, is described. Preparation of such gels is as easy and rapid as agarose gel preparation, and polymerized polyacrylamide gels are used to fractionate proteins or small DNA fragments using a common horizontal electrophoretic tank. This apparatus was used to electrophoretically fractionate proteins or DNA for immuno-blot analyses, particularirly in the study of the allergenic response to Parietaria judaica pollen in senescence, for Southern-blot hybridizations and in the study of DNA polymorphisms.
Highlights
Horizontal submerged agarose gel electrophoresis of nucleic acids is a basic tool of modern molecular biology [1]
We performed electrophoretic fractionation of proteins or DNA using gels prepared with our apparatus in an electrophoretic tank commonly used for agarose gel electrophoresis
Electrophoresis of proteins In order to verify the linear relationship between the logarithm of molecular mass and the relative mobility of proteins, we have fractionated different protein markers on horizontal polyacrylamide gels of different concentrations (Figure 2)
Summary
Horizontal submerged agarose gel electrophoresis of nucleic acids is a basic tool of modern molecular biology [1]. Proteins and very short nucleic acids are too small to be efficiently fractionated through the wide-pore agarose molecular sieve. Since a polyacrylamide solution does not polymerize in the presence of oxygen, its polymerization is obtained by loading the solution between two glass plates., placed vertically, and a plastic comb is inserted in the upper slot side to form the loading wells [2]. This procedure, which often needs a stacking gel on top of the polymerized polyacrylamide, is long and tedious compared to horizontal agarose gel preparation. Polyacrylamide polymerization set arrangement imply these gels to be run vertically, and vertical gels of different dimensions each need a purpose-built apparatus
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
