Abstract

The Direct Miscroscopic Somatic Cell Count — field method (DMSCC), Wisconsin Mastitis Test (WMT), and Electronic Somatic Cell Count (ESCC) were studied to determine variability and relationship to each other. The coefficients of variation computed at a DMSCC count near one million were 15.6% (DMSCC), 6.3% (WMT), and 4.2% (ESCC). Linear regression equations were determined for predicting DMSCC results by WMT and ESCC. The approximate width of the 95% confidence intervals for ESCC predicting DMSCC were ± 275,000 and for WMT predicting DMSCC were ± 600,000. The prediction of square root and log transformations of DMSCC by WMT exhibited narrower confidence intervals for low somatic cell counts, but wider intervals for high counts (greater than 1,000,000).

Highlights

  • The Direct Miscroscopic Somatic Cell Count - field method (DMSCC), Wisconsin Mastitis Test (WMT), and Electronic Somatic Cell Count (ESCC) were studied to determine variability and relationship to each other

  • The work was designed to evaluate variability of the three procedures, the DMSCC both at a level of one million and over the broad range of counts noted in commercial laboratories

  • The precision of the data presented in this paper when expressed as the coefficient of variation for counts near one million is 3.5o/o (ESCC-chemical method) 6.3o/o (WMT), and 15.6o/o (DMSCC-field method)

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Summary

Introduction

The Direct Miscroscopic Somatic Cell Count - field method (DMSCC), Wisconsin Mastitis Test (WMT), and Electronic Somatic Cell Count (ESCC) were studied to determine variability and relationship to each other. The chemical method of Electronic Somatic Cell Count utilizing a Coulter Counter (ESCC) is used as an official procedure in Canada and several European countries As yet, it has not been recognized as an official test in the U.S, but evidence of its applicability appears to be mounting (2, 3, 5-9) with a need perhaps only to refine details of operation. The DMSCC field method has not been analyzed for variability (as has the "reticle strip" procedure) but it is an official confirmatory test widely used throughout the U.S. The work reported was designed to further demonstrate the applicability of the ESCC to routine counting of somatic cells, and to compare it with and determine its relationship to DMSCC and WMT results. The work was designed to evaluate variability of the three procedures, the DMSCC both at a level of one million and over the broad range of counts noted in commercial laboratories

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