Abstract
Continuous wave (cw) and pulsed electron paramagnetic resonance (EPR) spectroscopy on spin-labeled membrane proteins provides information about the structure and dynamics of the biomolecule in the lipid bilayer or in membrane mimics such as nanolipoprotein particles (nanodiscs) and detergents. Here, the basic principles of EPR, site-directed spin labeling of proteins and cw, time resolved and pulsed EPR techniques for the analysis of the spin label side chain's mobility, its accessibility toward paramagnetic quencher molecules, the polarity of its direct environment, and the distance between two label side chains are described.
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