Abstract
The process of spore formation (ascosporogenesis) has not been clearly defined in ascomycete fungi. Many conflicting reports exist concerning details of ascospore initial development. The relatively slow fixation (up to several minutes) of conventional chemical techniques does not adequately preserve many structures for which ultrarapid freezing techniques (within milliseconds) are particularly suited. High-pressure frozen freeze-substituted samples of the ascomycete Sordaria humana were thick-sectioned for laser scanning microscopy (LSM) and thin-sectioned for transmission electron microscopy (TEM). Perithecia were too large (up to 500μm in diameter) to freeze adequately using plunge or propane-jet freezing techniques. The LSM was used to examine portions of the perithecia which were too large for viewing by TEM without taking numerous low magnification micrographs.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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