Electron microscopic alterations of the rat's pleura after experimental haemothorax

Abstract

The initial signs of pleural reactivity and the subsequent mechanisms of pleural healing still remain unsolved. The visceral and parietal (costal and diaphragmatic) pleura were investigated following an experimental haemothorax (EH) by transmission electron microscopy. Young-adult Wistar rats were divided in five groups and survived 6 hours, 1, 3, 8 and 15 days respectively after EH. Six hours after EH the mesothelial cells had a more prominent lysosomal system and electron-dense material in the vesicles, as in the dilatated intercellular spaces. On the 1st day of the EH the mesothelial cytoplasm formed a thin interrupted band. The extravasal cells built multiple layers over the basal lamina, leading to a thicker submesothelial layer, occupying the superficial position toward the pleural cavity. The activated mesothelial cells covered both pleural sheets on the 3rd day after EH. Eight days after EH different membrane bodies, large apical evaginations, elastic-like formations, an extensive vesicular and cytofilamentous systems characterized the mesothelium. The wider elastic membrane showed thickenings, protrusions, bifurcations and double course. Fifteen days after EH larger zones in both pleural sheets displayed thinner basal lamina, remnants of elastic membrane and a thicker submesothelial layer. In conclusion, different newly formed structures (reversible and stable) retain the tendency of enlargement of the pleural surface in all investigated periods. Simultaneous intercellular and transcellular transport, as an increase of the lysosomal system characterize the passing of the electron-dense material through the mesothelium. The early period (until 3rd day after EH) is characterized by more prominent mesothelial changes, involving activated cells. The initiation of the late period (on the 8th day after EH) begins with the appearance of lamellar bodies and newly formed elastic membrane. The following late changes (on the 15th day after EH) concern predominantly the components of the connective tissue layer, such as collagen accumulations and blood capillaries. The present data suggest that the alterations over the entire pleura are irregular and asynchronous, showing significant morphological differences in both pleura sheets, some of them are diffuse in character, the final ones appear to be stable and ensure incomplete pleural restoration.