Abstract
Electron holography has been applied to a variety of field, in which the reconstructed phase information was effectively used to elucidate many important natures of materials. Applications of electron holography to biological specimen, however, has not been developed yet. We report here an application of electron holography of frozen-hydrated biological specimen, which is observed by electron holography for the first time.An electron microscope(Hitachi HU-12A) equipped with a 100-kV cold field emission gun was used for the experiment. Catalase crystals were embedded in amorphous ice by quick-freezing and the grids were transferred to the microscope using Gatan cryo transfer system. A specially designed low-dose imaging system for the microscope was devised and used to record holograms.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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