Abstract

Electrokinetic transport mechanism through solid-state nanopores (SSNs) results from a complex interplay of electrophoresis, electroosmosis, and/or diffusion. These processes are influenced by a host of factors, viz., applied voltage, electrolyte type, concentration and pH, nanopore surface chemistry, analyte charge, etc. In this study, we investigated the transport phenomena associated with the translocation of protein and DNA. Unlike DNA, which has a uniform linear charge density, protein charge is heterogeneous and highly pH-dependent.

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