Abstract
In this work, an electrogenerated chemiluminescence (ECL) detector with improved stability was developed for high-performance liquid chromatography (HPLC) detection of hydrochlorothiazide (HCTZ). The detector was prepared by packing cation exchanged resin particles in a glass tube, followed by inserting Pt wires (working electrode) in this tube and sealing. The leakage of Ru(bpy)32+ from the resin was compensated by adding a small amount of Ru(bpy)32+ in the mobile phase. Factors affected the performance of the proposed ECL detector were investigated. Under the optimal conditions, the ECL intensity has a linear relationship with the concentration of HCTZ in the range of 5.0×10−8gmL−1−2.5×10−5gmL−1 and the detection limit was 2.0×10−8gmL−1 (S/N=3). Application of the detector to the analysis of HCTZ in human serum proved feasible.
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