Abstract
Electrofusion of mammalian cells is obtained by the pulsation of a cell suspension in a medium of low ionic content followed by the creation of contact between the pulsed cells. This process is linked to the induction of a long-lived fusogenic organization of the membrane by the external field. A large volume of cells can be fused by repetitively pulsing a cellular flow and by centrifuging the pulsed sample in order to create numerous contacts between the cells. The viability of the cells is not altered by this treatment.
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