Abstract
Direct electron transfer of hemoglobin (Hb) in the chitosan (CTS) and <TEX>$TiO_2$</TEX> nanoparticles (nano-<TEX>$TiO_2$</TEX>) composite films was achieved by using a room temperature ionic liquid of 1-butyl-3-methylimidazolium hexafluorophosphate (<TEX>$BMIMPF_6$</TEX>) modified carbon paste electrode (CILE) as the basal electrode. UV-Vis and FT-IR spectroscopy indicated that Hb in the film retained the native structure. Electrochemical investigation indicated that a pair of well-defined quasi-reversible redox peaks of Hb heme Fe(III)/Fe(II) was obtained with the formal potential located at -0.340 V (νs. SCE) in pH 7.0 phosphate buffer solution (PBS). The electrochemical parameters such as the electron transfer coefficient (α), the electron transfer number (n) and the standard electron transfer rate constant (<TEX>$k_s$</TEX>) were got as 0.422, 0.93 and 0.117 <TEX>$s^{-1}$</TEX>, respectively. The fabricated CTS/nano-<TEX>$TiO_2$</TEX>/Hb/CILE showed good electrocatalytic ability to the reduction of trichloroacetic acid (TCA) and hydrogen peroxide (<TEX>$H_2O_2$</TEX>), which exhibited a potential application in fabricating a new kind of third generation biosensor.
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