Abstract
The interaction of copper-1,10-phenanthroline (phen) complex with calf thymus DNA has been investigated by cyclic voltammetry and differential pulse voltammetry at gold electrode. DNA can be efficiently cleaved by electrochemically reduced Cu(phen) complex at −0.5 V (vs. Ag/AgCl) in the presence of oxygen without addition of extra reductive agent. Oxygen is required for electrochemically induced DNA cleavage by Cu(phen) complex because it serve as a precursor formation of hydrogen peroxide. DNA cleavage is inhibited by bathocuproine and hydroxyl radical scavenges. These results reveal Cu(I) complex and hydroxyl radical are involved in DNA cleavage reaction. The percentage of DNA cleavage is enhanced with increasing electrolysis time and is also dependent on the ratio of Cu(phen) to DNA concentration. The cleaved DNA fragments are separated by high performance liquid chromatography (HPLC). The experimental results indicate that the method for electrochemically induced DNA cleavage by Cu(phen) is simple and efficient.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
