Abstract
A flow-electrolytical cell containing a strand of micro Au modified carbon fiber electrodes (CFE) has been designedand characterized for use in a voltammatric detector for detecting cysteine using high-performance liquid chromatography. Cysteine is more efficiently electrochemical oxidized on a Au /CFE than a bare gold and carbon fiber electrode. The possible reaction mechanism of the oxidation process is described from the relations to scan rate, peak potentials and currents. For the pulse mode, and measurements with suitable experimental parameters, a linear concentration from 0.5 to 5.0 mg·L−1 was found. The limit of quantification for cysteine was below 60 ng·mL−1.
Highlights
The sulfhydryl (-SH) group of cysteine plays a key role in the biological activity of proteins and enzymes
In order to improve the selectivity and sensitivity of determination of cysteine, alternative methods such as high-performance liquid chromatography or flow injection with pulsed electrochemical detection employing a gold working electrode have been published in the literature [15,16,17,18]
These electrodes were constructed for capillary electrophoresis [24,25,26,27,28], liquid chromatography [29,30] to detect amino acids
Summary
The sulfhydryl (-SH) group of cysteine plays a key role in the biological activity of proteins and enzymes. In order to improve the selectivity and sensitivity of determination of cysteine, alternative methods such as high-performance liquid chromatography or flow injection with pulsed electrochemical detection employing a gold working electrode have been published in the literature [15,16,17,18]. These electrodes were constructed for capillary electrophoresis [24,25,26,27,28], liquid chromatography [29,30] to detect amino acids. In this study we describe the construction of a disposable electrode sensor, composed of gold deposited on a carbon fiber substrate, for the high-performance liquid chromatography and the pulsed amperometric detection of cysteine
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