Abstract
Tethered lipid bilayer membranes (tBLM’s) are biomimetic model membranes, that enable protein reconstitution. Submembrane reservoir that separates lipid bilayer from solid support prevents reconstituted proteins from jamming against solid surface. However, this makes tBLM’s intrinsically asymmetric, bathed in ∼1.5-2 nm electrolyte reservoir from one side and practically infinite electrolyte layer from the other side. This asymmetry hinders possibility to measure conductance of ion channels. On the other hand, this asymmetry allows to estimate lateral organization of ionic channels without utilizing microscopic techniques.
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