Abstract
An electrochemical immunosensor for the detection of protein biomarkers is reported herein based on using magnetic nanoparticles (MNs) as labels. Reaction of potassium ferrocyanide with MNs leads to in situ formation of Prussian blue (PB) on the MN surface, generating an electrochemical current. A carcinoembryonic antigen (CEA) was chosen as a model protein biomarker to test the performance of the immunosensor. The immunosensor was prepared based on a traditional sandwich-type structure. A high concentration of CEA increased the number of MNs captured on the electrode, which increased the amount of PB produced, thus increasing the electrochemical current intensity. The current intensity linearly increased with the concentration of CEA in the range from 0.05 to 10 ng/mL. The potential clinical applicability of this immunosensor was demonstrated by CEA detection in human serum samples.
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