Abstract
Diamine oxidase (DAO) has been examined in order to obtain a sensor for biogenic amines; the enzyme was immobilised directly on the surface of a platinum electrode, poised at +700 mV vs. Ag/AgCl, or immobilised on glass beads in a small reactor (diameter 2 mm, length 40 mm) and included in a flow-injection analysis assembly. Moreover, it was coupled with horseradish peroxidase coimmobilised and coupled with a glassy carbon electrode using ferrocene monocarboxylic acid as mediator dissolved in the carrier stream. Low detection limits were obtained in all cases with some differences; the selectivity was quite similar in the different assemblies. Moreover, we showed that selectivity can be changed using DAO, obtained from different starting materials not commercially available such as pea ( Cicer arietinum).
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