Abstract
A universal strategy was developed for the analysis of circulating tumor cells (CTCs) based on reaction of DNA in the cells with molybdate. Initially, CTCs were enriched and isolated from samples by magnetic nanoparticles. Then, after killing the isolated cells by heat treatment, the cell membrane was raptured, and the DNA molecules contained in the cells were released. The following reaction of the released DNA molecules with molybdate can form redox molybdophosphate, resulting in electrochemical current. This electrochemical assay can be applied to thedetection of different CTCs as long as the CTCs can be isolated from thesamples, with a universal signal detection method, without additional signal amplification strategies. Breast cancer cell MCF-7 was chosen as a model CTC for thisstudy. At aworking potential of 0.2V vs. Ag/AgCl electrode, the electrochemical current is linearly related to theMCF-7 cell concentration from 5 to 1000 cellsmL-1 witha limit of detection of 2 cellsmL-1. The assay was successfully appliedfor detection of MCF-7 in human blood samples. This electrochemical assay can be applied for detection of different CTCs and alsofor simultaneous detection of CTCs. Graphical abstract A universal strategy was developed for the analysis of circulating tumor cells (CTCs) based on reaction of DNA contained in the cells with molybdate.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
