Abstract
In this study, Mortierella alpina and Mortierella elongata strains were compared for their growth, lipid accumulation and eicosapentaenoic acid (EPA) yield in different media. M. elongata (NRRL 5513) was found to give highest EPA yield. It grew well at 11° as well as at 25 °C in a liquid medium containing glucose or linseed oil and yeast extract. M. alpina strains did not produce EPA at 25° in the absence of linseed oil and M. alpina ATCC 32221 produced no EPA even in the presence of linseed oil. M. elongata (NRRL 5513) mycelium containing EPA 19·7 mg g−1 (dry weight) yielded 0·473 g l−1 EPA constituting 5·8% of the lipid fraction when it was grown at 11° for 10 d in a medium containing 2% linseed oil as carbon source. EPA production by M. elongata was highest when glucose concentration in the medium was low.
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