Abstract
Disruption of the intestinal barrier is closely associated with intestinal inflammation and disease development. Ovomucin is a bioactive protein in chicken egg white, and its hydrolysate has been revealed to prevent the adhesion of pathogen to intestinal epithelial cells. However, the effect of ovomucin hydrolysate on intestinal barrier integrity and associated anti-inflammation mechanism has not been demonstrated. Here, we investigated its activity in lipopolysaccharide (LPS)-induced monolayer dysfunction of differentiated Caco-2 cells to unravel the underlying molecular mechanisms. Ovomucin-protex 26L hydrolysate significantly increased transepithelial electronic resistance (TEER) values, decreased the paracellular FITC-dextran flux permeability, and recovered the expression of occludin and ZO-1 in LPS-stimulated Caco-2 cells. Meanwhile, ovomucin-protex 26L hydrolysate significantly inhibited the LPS-induced activation of NF-κB and MAPK pathways. These results suggested the ability of ovomucin-protex 26L hydrolysate to suppress LPS-induced intestinal barrier function damage via the inhibition of inflammatory responses.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
