EGFR gene copy number in adenocarcinoma of the lung by FISH analysis: Investigation of significantly related factors on CT, FDG-PET, and histopathology

Abstract

It has been suggested that a high EGFR gene copy number may be an indicator of good response to EGFR tyrosine kinase inhibitor therapy and a marker of poor prognosis in NSCLC. However, imaging features related to EGFR gene copy number status in adenocarcinoma are still unknown. We therefore retrospectively analyzed CT, FDG-PET, and histopathologic slides of surgical resected lung adenocarcinoma in 132 patients. Tumor characteristics on preoperative chest-CT, such as, GGO proportions, tumor diameters, and cavitation; FDG-PET SUV max; and histopathologically determined differentiation degrees and tumor subtypes were evaluated. EGFR gene copy number status was categorized as FISH-positive or -negative. FISH-positivity was found in 53 patients (40.2%) and was significantly more frequent in tumors with a SUV max > 7.0 ( P = 0.007). Furthermore, FISH-negativity was found to be more frequent in tumors with a GGO > 50% ( P = 0.023) and diameter <15.5 mm ( P = 0.006) on CT, or a well-differentiated histopathology ( P = 0.002). Moreover, the frequency of FISH-positivity increased as SUV max increased ( P = 0.0008) and as the proportion of GGO decreased ( P = 0.01). SUV max > 7.0 was an independent predictor of FISH-positive results (odds ratio, 3.941; 95% CI, 1.691–9.182; P = 0.01). In conclusion, a high SUV max on FDG-PET was significantly related to FISH-positive results. A high proportion of GGO, small tumor diameter on CT, and a well-differentiated histopathology were more frequent in FISH-negative adenocarcinomas.