Abstract
An efficient induction of protocorm-like bodies (PLBs) from seedlings of dwarf Dendrobium was accomplished in order to develop mass-scale propagation. The effect of N6-benzyladenine (BA) and thidiazuron (TDZ) on the in vitro induction of PLBs was studied. TDZ was found to be a more effective inducer of PLBs and their proliferation than BA. The highest percentage for the induction of PLBs (86%) and the highest number of PLBs (3.6) per protocorm were observed after 9 weeks of culture in modified Murashige and Skoog (MS) liquid medium supplemented with 18 ?M TDZ.
Highlights
Orchids are popular horticultural and ornamental plants mostly because of their exquisite flowers
The protocorms developed into protocorm-like bodies (PLBs) after 6 weeks of culture on the PLB induction media
The efficiency of protocorms producing PLBs was dependent on the types and concentrations of cytokinins in the medium
Summary
Orchids are popular horticultural and ornamental plants mostly because of their exquisite flowers. Sexual propagation to produce complete plants through orchid seeds is difficult because their seeds are minute and have no endosperm. They need symbiotic fungi in order to germinate (Anjum et al, 2006; Thomas and Michael, 2007). Its flowers have longevity and blooms many times a year It is generally used as a flowering-potted for ornamental plants. It has potential commercial value as gifts, souvenirs, or decorations which are miniaturized orchid plantlets with flowers. Tissue culture has for many years played an important role as a means to propagate orchids and several in vitro cultural protocols have been developed in this genus (Arditti and Ernst, 1993; Chugh et al, 2009)
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