Abstract
Estimating pollen viability is important for analyzing genetic flow, evaluating the masculine reproduction potential of a species, and for genetic improvement programs. In order to gather more information about different pepper accessions (Capsicum sp) held in the Active Germplasm Bank of the Federal University of Piauí (BAG-UFPI), Brazil, we analyzed the pollen viability of eight pepper accessions using four colorimetric methods: 2% acetic carmine; 2% acetic orcein; fuchsin; and Lugol’s iodine. Pre-anthesis floral buds were collected and fixed in ethanol:acetic acid (3:1) for 24 hours and subsequently held in 70% ethanol under refrigeration until the slides were prepared using the squashing technique. An entirely randomized methodology was used, and the data compared using the Scott-Knott test at a 5% probability level. The four colorimetric methods were found to be efficient for estimating pollen viability in the different plant accessions, with all of them demonstrating high pollen viability (above 70%) – an important index for studies of genetic improvement.
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