Abstract
Objective: The objective of the present study is the evaluation of the effect of the sublethal (40% and 60% of 48h LC50) binary combination (1:5 ratios) of molluscicides deltamethrin+MGK-264 on the endogenous levels of protein, amino acid and nucleic acid in different tissues of snail Lymnaea acuminata.
 Methods: The snails were treated with 1:5 mixtures of sub-lethal concentration of (40% and 60% of 48h LC50) deltamethrin+MGK-264 on the protein, amino acid and nucleic acid levels in gonadal, nervous and foot tissue of L. acuminata. In order to study the effect of withdrawal from treatment, the snails were first exposed to the above concentrations for 96h, after which they were transferred to freshwater. Water was changed every 24h for the next seven days, after which different biochemical parameters were estimated.
 Results: There was a significant change in the levels of protein (sublethal concentration of 60% of 48h LC50 after 96h) gonadal, nervous and foot tissues are 48.1, 12.1 and 14.5%, respectively, amino acid are 273, 234 and 252%, respectively, DNA are 25.1, 38.9 and 42.1%, respectively and RNA are 12.2, 30.7 and 30.5%, respectively. These changes were time and concentration-dependent. In the withdrawal experiment, the snails were treated for 96h to transfer in freshwater for 7 d, which caused significant recovery in all the biochemical parameters.
 Conclusion: The present study concluded that the high molluscicidal activity of deltamethrin+MGK-264 simultaneous decrease in the levels of proteins, DNA, RNA and increase in the level of amino acids.
Highlights
Digenetic trematode Fasciola gigantica is a parasite of great importance in eastern Uttar Pradesh, which causes endemic fasciolosis in cattle and livestock in northern part of India [1]
In snail treated with 40% of LC50 for 96h the total protein levels which have decreased to 63.1%, 29.2% and 22.21% of controls in the gonadal, nervous and foot tissue recovered to 95.52%, 97.92% and 98.44% of the controls in corresponding tissues
After seven days withdrawal period of snails treated with 60% of 48h LC50 for 96h, the total protein levels in gonadal, nervous and foot tissue recovered to 93.64%, 94.47% and 93.26% of controls, respectively
Summary
Digenetic trematode Fasciola gigantica is a parasite of great importance in eastern Uttar Pradesh, which causes endemic fasciolosis in cattle and livestock in northern part of India [1]. Indiscriminate use of synthetic pyrethroids against the different pests control has created the problem of acute and chronic toxicity to man and other non-target animals [9]. As a group synthetic pyrethroids causes high pest mortality at lower concentration [17,18,19] and are less toxic to the non-target animal, especially mammal [20]. Singh et al [4] has studied the effect of sublethal, and deltamethrin binary combination of deltamethrin+MGK-264 on the levels of phospholipids and lipid peroxidation in the nervous tissue of snail L. acuminata. Present study is the continuation of the same work to observe the effect of the sub-lethal binary combination of deltamethrin+MGK-264 on the endogenous levels of protein, amino acid, nucleic acid in different tissues of the snail L. acuminata
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