Efficacy of adrenomedullin on cisplatin ototoxicity in rats

Abstract

Purpose: The aim of this study was to evaluate the efficacy of adrenomedullin on damage associated with cisplatin ototoxicity created in an experimental animal model.Materials and Methods: A total of 32 Wistar Albino rats were separated into 4 groups of 8. To the rats in Group 1 (control), 1% acetic acid was administered intraperitoneally and intratympanically, to Group 2, cisplatin (10mg/kg/2 days) + intratympanic acetic acid, to Group 3, cisplatin + intraperitoneal adrenomedullin (12 µg/kg) and to Group 4, cisplatin +0.1- 0.3ml intratympanic adrenomedullin (12 µg/kg). The drugs were administered on the first 5 days and signal-to-noise ratio (SNR) measurements of the auditory brainstem response (ABR) and distortion product otoacoustic emissions (DPOAE) were made on days 0, 5 and 15. The rats were sacrificed and serum and ear tissue samples were taken for histopathological examination and examination of oxidative stress levels.Results: A statistically significant difference was determined in the cisplatin+intratympanic adrenomedullin group in the Auditory brain-stem response (ABR) measurements on days 0, 5 and 15 . In the Distortion product-evoked otoacoustic emission (DPOAE) measurements, the between-day differences at 16000 frequency in the cisplatin + intraperitoneal adrenomedullin group were accepted as statistically significantConclusion: Adrenomedullin, which is a strong vasodilator and antioxidant, was found to have a protective effect against cisplatin ototoxicity in the cochlea.