Abstract
The oxidation of lignin peroxidase (LiP) of Phanerochaete chrysosporium was examined using various phenolics and aromatic amines as substrates in organic solvents. LiP oxidized 3,3′-dimethoxybenzidine more effectively in several 70% aqueous water-miscible organic solvents including ethylene glycol and methylcellosolve than in water. LiP activity in water-miscible organic solvents was correlated with the Dimroth-Reichardt parameter ( E T (30)) of the solvents, which is directly related to free energy of the solvation process. In addition, absorption spectra of LiP in several glycols were similar to those in succinate buffer. These results suggest that these glycols do not disturb the conformation around the active center, heme, of LiP. Furthermore, LiP catalyzed the oxidation of several phenolics and aromatic amines including 3,3′-dimethoxybenzidine, o-, and p-phenylenediamines and o-aminophenol in 70% aqueous ethylene glycol solution. These substrates were shown to have low ionization potential and high hydrophobicity, and thus to be suitable substrates for the reaction of LiP in water-miscible organic solvents.
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