Abstract
To improve productivity in the cultivation of Chinese yam 'Tanba-yamanoimo' (Dioscorea opposita Thunb.), we investigated the effect of virus free plants on the yield of a high yielding variety 'Shintanmaru' at a commercial field in 1998, 1999 and 2000. The weights of rhizophore of the virus free clone obtained from shoot apex culture were significantly heavier than infected plants with Japanese yam mosaic virus. The rates of yield increase were 27% in 1998, 17% in 1999 and 23% in 2000. The infected plants with Japanese yam mosaic virus showed the disease symptoms as mosaic in leaf, leaf transformation, leaf miniaturizing, and a delay in sprouting and growth. They could be easily distinguished from the virus free plants by their appearance. The results of distinction by symptoms in appearance and by serum test were nearly the same, with few exceptions. Because the former method was simple and not costly, it was a more effective and practical way to obtain virus free plants in seed propagation.
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