Abstract
PURPOSETo determine if a relationship exists between physical fitness and the ability of CD34+ or CD34−/CD31+ circulating angiogenic cells (CAC) to influence angiogenic function through paracrine activity.METHODSPeripheral blood was drawn from healthy inactive (n = 7), active (n = 10) and endurance‐trained (n = 7) men and women aged 25–39. Mononuclear cells were isolated by density centrifugation and CD34+ and CD34−/CD31+ fractions were purified by immuno‐magnetic selection. Conditioned media (CM) from cultured CACs was retrieved and used for a HUVEC‐based angiogenesis tube formation assay. Tube length and complexity were imaged and quantified.RESULTSCM from CD34+ CACs of endurance‐trained subjects resulted in 21% greater tube length (P<0.05) and 48% greater complexity (P<0.05) than inactive subjects, and 22% greater complexity than active subjects (P<0.05). CD34+ CM from active subjects produced greater length and complexity than inactive subjects (P<0.05, 18% and 33%, respectively). Tube length was 22% greater and complexity was 42% greater (P<0.05 each) with CD34−/CD31+ CAC CM of endurance‐trained subjects compared to inactive individuals.CONCLUSIONSThese preliminary data provide promising support for a chronic exercise‐induced increase in paracrine‐mediated angiogenic function of CD34+ and CD34−/CD31+ CACs.Support: NIH AGT3200068 and UMD KNES Graduate Research Initiative Fund
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