Abstract

In germination tests, the trichothene diacetoxyscerpanol (DAS) caused reductions in the number and total length of rootlets, and the length of the coleoptile, developing from excised barley embryos, but the effects were less than those of T-2 toxin. Rootlet and coleoptile growth was also inhibited during micromating of barley artificially contaminated with DAS before steeping, whilst α-amylase activity of finished malts were reduced by up to 49%, and α-amino nitrogen levels in worts were also lower than in controls. The effects of deoxynivalenol (DON) were less than those of DAS, e.g. the reduction in α-amylase activity caused by DON applied at the rate of 50 μg g-1 grain was less than one-half of that caused by 20 μg DAS g−1.

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