Abstract

Abstract Embryos of tomato (Lycopersicon esculentum Mill.) were excised 15 days after pollination and cultured on 20 different media to test a broad range of concentrations of salts, sucrose, and organic supplements on growth and development. Embryo growth and morphological development on 6 of the experimental media were better than on Murashige and Skoog’s (1962) revised tissue culture medium. Salt and sucrose levels were significant in their effects on growth, as was the interaction of salts with vitamins and amino acids. The medium with high salts, high sucrose, and low vitamins and amino acids was superior to any of the other treatments.

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