Abstract

Using apple pomace (AP) as substrate, a series of batch experiments were conducted to investigate the effects of activated sludge pretreated by ultraviolet and ultrasonic on the anaerobic fermentative bio-H(2) production. The results show that a maximum cumulative H(2) production (CHP(m)) of 107.0mlg(-1)-TS and an average H(2) production rate (AHPR) of 15.0mlg(-1)-TSh(-1) were obtained by using the pretreated sludge that was irradiated by ultraviolet lamp (25w) for 15min as inoculant. Based on the optimal sludge pretreatment method, the experiments of bio-H(2) production under the optimal substrate pretreatment condition (soaked in the ammonia liquor of 6% for 24h) were further carried out, and the CHP(m) of 138.8mlg(-1)-TS was achieved, increased by 80.6% compared to the un-pretreated group (76.8mlg(-1)-TS). The effects of the various organic acids on the fermentative H(2) production were also investigated under the above optimal sludge pretreatment condition.

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