Abstract
Sleep deprivation (SD) increase catabolic hormone concentrations and decrease growth factors, which may impair the muscle regeneration. PURPOSE: Evaluate the influence of SD on the muscle regeneration, cellular proliferation and muscular insulin-like growth factor-1 (IGF-1) concentration after cryolesioning in rats. METHODS: Forty male rats, Wistar, 3 month, were distributed into 4 groups: Control (CTL), submitted to SD for 96h (SD96), CTL plus sleep recovery period (CTL+R), submitted to SD for 96h plus 96h of sleep recovery (SD96+R). Previously, the animals were submitted to cryolesioning of Tibialis anterior (TA) from left leg. After 3 days of recuperation, SD96 and SD96+R groups were sleep deprived for 96h by modified multiple platform method, following of sleep recovery for 96 h to SD96+R group. TA of both legs were excised (intact and injured) to histopathologic analysis (with HE, 40x), PCNA (western blot) and muscular IGF-1 (ELISA) analysis. The data were analyzed by one way ANOVA. RESULTS: The concentration of IGF-1 in intact leg was reduced in SD96 compared to CTL (356±27 vs 816±105 ng/dL respectively; P<0.05), and the basal values were reestablished in SD96+R group (776±69 ng/dL). In injured leg, the concentration of IGF-1 increased in all groups, but with less magnitude in SD96 compared to CTL (1442±256 vs 3851±249 respectively; P<0.001) and SD96+R compared to CTL+R (1605±260 vs 2307±182 ng/dL respectively; P<0.001). PCNA protein not change with SD, but injury increased its concentration proportionally in all groups when compared to intact leg (CTL - 1.88±0.22 vs 0.46±0.13 AU respectively; SD96 - 2.4±0.6 vs 0.62±0.12 AU respectively; CTL+R - 1.68±0.35 vs 0.55±0.08 AU respectively; SD96+R - 1.4±0.13 vs 0.71±0.05 AU respectively; P≤0.05 for all). The qualitative histopathologic analysis showed a delay in process of muscle regeneration, with higher amounts of fibrous tissue, inflammatory process and muscle atrophy in SD96+R group compared to CTL+R. CONCLUSION: SD reduced muscular IGF-1 concentration and delayed the muscle regeneration stimulated by cryolesioning in TA of rats. Supported by CEPID/SONO-FAPESP (#98/14303-3), CNPq, CAPES, and FAPESP (#2011/15962-7; 2013/00152-5)
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