Effects of restricted fluid resuscitation on expression of liver nitric oxide synthase in rats with hemorrhagic shock

Abstract

Objective To investigate the effects of restricted fluid resuscitation on expression of liver nitric oxide synthase in rats with hemorrhagic shock. Methods A model of rat with severe hemorrhagic shock caused by active bleeding was established in 60 SD rats.Those rats were randomly divided into control group, restricted group (restricted fluid resuscitation group) and conventional group (conventional large-volume fluid resuscitation group). After different treatments, survival rats in each group were detected and compared of NO in serum, the expressions of NOS mRNA and protein respectively. Results NO in serum, the mRNA and protein expressions of eNOS in liver of restricted group were obviously higher than those of conventional group(P<0.05). Compared with control group, NO in serum, the mRNA and protein expressions of eNOS in liver of conventional group were significantly increased(P<0.05). The mRNA and protein expressions of iNOS in liver of conventional group were significantly lower than those of control group(P<0.05). Compared with conventional group and control group, the mRNA and protein expressions of iNOS in liver of restricted group were obviously decreased(P<0.05).Conclusions Restricted fluid resuscitation can significantly protect liver from microcirculation disorder injury. The mechanism might be related with the induction of eNOS in tissue and the increase of NO in serum. Key words: Restricted fluid resuscitation ; Hemorrhagic shock ; Nitric oxide synthase ;