Abstract
Background/aimPhenytoin is an anticonvulsant drug which causes fibroblast proliferation, collagen synthesis, and an increase in epidermal growth factor. Therefore, the aim of the present study is to evaluate the effect of phenytoin injection on the wound healing process in rats with vocal cord injury by histopathological methods.Materials and methodsThe vocal cords of 10 albino Wistar rats were damaged bilaterally; the left vocal cord was kept as the control group. Phenytoin was injected in the right vocal cord. Ten rats were sacrificed. The thickness of the lamina propria and density of the fibroblast and collagen were evaluated histopathologically.ResultsThickness of the lamina propria was 18.0 ± 7.1 µm in the control group, 65.5 ± 10.7 µm in the phenytoin group. The density of fibroblast and collagen were statistically lower in the control group compared the phenytoin group (P < 0.05).ConclusionPhenytoin injection in rats after vocal cord injury significantly increased the thickness of the lamina propria and density of fibroblast and regular and mature collagen in the lamina propria. The findings in our study provide a feasible scientific view for adding phenytoin treatment to vocal cord surgeries in otolaryngology practice, but further studies are needed in order to evaluate the use of phenytoin in preventing the formation of scar tissue and possible effects on vocal cord vibration in humans after vocal cord injury.
Highlights
Vocal cord injury is a significant problem which affects quality of life and causes severe voice disturbances [1]
The density of fibroblast and collagen were statistically lower in the control group compared the phenytoin group (P < 0.05)
Phenytoin injection in rats after vocal cord injury significantly increased the thickness of the lamina propria and density of fibroblast and regular and mature collagen in the lamina propria
Summary
Vocal cord injury is a significant problem which affects quality of life and causes severe voice disturbances [1]. Scar tissue usually develops after surgery, radiotherapy, and inflammation [2]. This leads to decreased viscoelasticity of the vocal fold lamina propria (increased stiffness), which reduces the vibration potential of the vocal folds [3]. There are no recent effective treatments for preventing vocal cord injury. The treatment is usually difficult and may include voice therapy with a speech and language therapist and injection augmentation. In order to discover an effective treatment modality, events occurring in the early stages after vocal cord injury should be known. The efficacy of these treatments is usually limited; these treatments are unable to recover the normal distribution of extracellular matrix components [7,8,9,10]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
