Abstract
Objective To discuss the effects of Protocadherin 11 X-linked protein (Pcdh11x) on formation of neuronal dendritic branching. Methods In this study, using gain-of-function and loss-of-function studies, we count the number of neural dendritic branching, and analyzed the data with SPSS 13.0 software by one way ANOVA. Results Compared with the control GFP vector, Pcdh11x-shRNA increased, whereas pCAG-Pcdh11x-GFP decreased the number of primary and secondary dendrites. The number of dendritic tips was reduced in neurons overexpressing pCAG-Pcdh11x-GFP, whereas it was increased when Pcdh11x activity was inhibited by overexpression of Pcdh11x-shRNA. The effects of Pcdh11x-shRNA and pCAG-Pcdh11x-GFP on dendritic branching were confirmed by Sholl analysis. Therefore pcdh11x acts as a negtive regulator of dendritic branching in cultured cortical neurons from embryonic day 16 mouse embryos. Conclusions Pcdh11x Negatively Regulates Dendritic Branching, including primary dendrites, secondary dendrites, dendritic tips and crossing number. Key words: Pcdh11x; Central nervous system; Dendritic branching
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
