Effects of nutrient solution electrical conductivity and sulfur, magnesium, and phosphorus concentration on sesquiterpene lactones in hydroponically grown lettuce ( Lactuca sativa L.)

Abstract

The effect of nutrient solution electrical conductivity (EC) and sulfur (S), magnesium (Mg), and phosphorus (P) levels on the content of the primary sesquiterpene lactones (SLs), lactucin, 8-deoxylactucin, and lactucopicrin, in hydroponically grown lettuce was assessed. Lettuce grown at 4 EC levels (0.5, 1.0, 2.0, and 3.0 dS m −1) displayed significant differences in leaf area index, number of leaves, plant height, fresh weight per plant, and chlorophyll content that were highest at EC 2.0 dS m −1. Lactucin (5.5 μg g −1 dry weight), 8-deoxylactucin (7.5), lactucopicrin (35.8), and total SLs (48.7) concentraions were highest at EC 0.5 dS m −1. Four S (8, 16, 48, and 80 mg L −1) and Mg (6, 12, 36, and 60 mg L −1) levels and 3 P (8, 16, and 48 mg L −1) levels were assessed for their effect on individual and total SLs. S and P had the greatest effect on SL levels. Plants in the lowest S level had significantly higher lactucin, lactucopicrin and total SLs. Each of the SLs was higher in the highest P level while Mg influenced only the lactucopicrin level in a quadratic manner. The results indicate that solution culture conditions can strongly influence the SL concentration and therefore bitterness and acceptability of lettuce.