Effects of neuron-conditioned medium and fetal calf serum content on glial growth in dissociated cultures

Abstract

The influence of the microenvironment as assessed by medium conditioned by 6-day-old chick embryo neurons in culture and of the nutrients derived from fetal bovine serum was evaluated in cultures of primary chick embryo glial cells. Glia-enriched cultures from 15-day-old chick embryo were incubated from culture days 3–9 with various concentrations of neuron-conditioned medium, with or without 10% fetal bovine serum in the final culture medium. Also, glial growth was studied in cultures with 5%, 10% or 20% fetal bovine serum in the medium. Glutamine synthetase and 2′,3′,-cyclic nucleotide 3′-phosphohydrolase were used as astrocytic and oligodendrocytic markers, respectively. Cultures were harvested at day 9. The presence of neuron-conditioned medium in the cultures was associated with persistence of immature glioblast-like cells. This persistence of glial immature cells was also reflected by the lower glutamine synthetase activity in the cultures with neuron-conditioned medium as compared to cultures with neuron-conditioned medium and fetal calf serum. In cultures with 5% neuron-conditioned medium without fetal bovine serum, cyclic nucleotide phosphohydrolase activity was increased. We are assuming that the input of neurons to the microenvironment is partially mediated through the neuron-conditioned medium. Thus, the present findings show that neurons influence the growth and differentiation of glial cells in culture.